Analysis Note

ControlMouse DA-1a cells

Application

Research CategoryStem Cell Research

Immunoblotting: a starting dilution of 1-10 µg/mL is suggested.

EIA

Optimal working dilutions must be determined by the end user.

Anti-Mouse Leukemia Inhibitory Factor Antibody, clone 2H2.2 is an antibody against Mouse Leukemia Inhibitory Factor for use in ELISA & WB.

Research Sub CategoryPluripotent & Early Differentiation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Leukemia inhibitory factor, or LIF, is a pleiotropic cytokine that is expressed by a wide variety of cells including activated T lymphocytes, monocytes, mast cells and neuronal cells. Human LIF cDNA encodes a 202 amino acid precursor which is processed to yield a 180 amino acid, biologically active glycoprotein with an approximate molecular weight of 38-67 kDa. In addition to its involvement in the stimulation of acute-phase protein during inflammatory processes, LIF has also been implicated in promoting the survival and growth of axons in vitro. LIF signals through the LIF receptor, a heterodimer comprised of a 190 kDa LIFR subunit and the gp130 subunit common to the IL-6, IL-11, CNTF and OSM receptors. Janus tyrosine kinases, JAK1 and JAK2, are activated in response to LIF stimulation, which in turn phosphorylate Stat1 and Stat3 causing their nuclear translocation and the transcription of Stat-responsive genes.

Immunogen

Recombinant Murine LIF.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25M NaCl ph 7.6 with 0.1% Sodium Azide.

Specificity

This antibody is reactive to E.coli and STO derived recombinant murine Leukemia Inhibitory Factor (LIF).

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.